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After the rain, puddles everywhere. They quickly turn green, smelly : probably living organisms modifying this short-lived ecosystem
Plenty of water, yes, but what are the living organisms ? Are stagnant water ecosystems always the same ?

To find out, we created 2 artificial puddles, put them in different environmental conditions and compared their physical and biological characteristics.
I - Material and methods
A - Our artificial puddles
B - Physical measurements
1- pH
2- Temperature
3- Turbidity
C - Microscopy

II - Results and discussion
A - Physical characteristics
1- pH
2- Temperature
3- Turbidity
B - Biological characteristics
1 – Diatoms (by Lou-Anna/Eva and Arnaud/Hugo)
2 - Vessels elements (by Titouan/Naël and by Marion/Lou V.)
3 - Rotifera (by Aline/Juliette and by Quentin/Thibaud )
4 - Paramecia (by Rose/Lou P)
puddle

Rotifera (Quentin and Thibaud)

Using the microscope, we saw a creature eating some leaves of lettuce. It was transparent and nearly 0.1-0.5 mm long. It was a rotifer. It is a microscopic aquatic animal which can be found in freshwater environments like lake bottoms, or flowing water environments such as rivers or streams.

Rotifers have a long body made of three sections: a head, a body, and a foot. The foot usually has two toes. The head of a rotifer has a large, mouth, surrounded by cilia. The rotifer's cilia are used to take food and to move in the water.
Rotifers are not favored for fossilization because of their very small size.  They are mostly used in aquaculture and aquariums because of their very high reproductive rates.

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Rotifera (Aline and Juliette)

The rotifers are little organisms with bilateral symetry. They have a side between 50 micrometers and 3 millimeters. They have lashes around their mouse and these lashes make them move forward. Some rotifers are free swimming, others move by inchworming along a substrate, and some are sessile, moving inside tubes or gelatinous holdfasts that are attached to a substrate. Rotifers are an important part of freshwater zooplanktons being a major foodsource and with many species also contributing to the decomposition of soil organic matter.

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the Vessels elements (Titouan and Naël)

The vessel element from lettuce

A vessel element is a little spiral shaped object in the wall of the vessel that carry sap and water in the plant. The vessel element is made of lignin the same molecule that composed the wood.It’s utility is to prevent the vessel from collapsing on itself,because the lignin make it very hard. When the lettuce die,some little organisme start eating the lettuce and the vessel,and only left the hardest part,the vessel element.

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Vessel elements (Marion and Lou V)

In the stagnant water, we can find remains from the vessel elements from the lettuce. Vessel elements are cells bound together to transport water with dissolved minerals, from the root to the leaves. They are like are blood vessels, and they are a part of the xylem (a type of transport tissue in the plant). These cells are strengthened by lignin, the principal component of wood. The lignin is arranged in spiral which makes the vessel stronger.

IMG_2816

Vessel elements examined with an optical microscope

What is left in the stagnant water is the hard lignin spiral, as the soft components of the cell have been eaten by microorganisms.

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Paramecia (Rose LJ and Lou P.)

Paramecia is a living organism belonging to the large named the ciliated protozoan. His size ranges from 100 to 300 microns. This unicellular animal uses his numerous cilia to move and feed off bacteria and small algae.

The paramecium is a fresh water organism found in abundance in ponds, small and slow streams, or biofilms at the surface of puddles and dead plants. The cell nucleus is divided in two parts, a small micronucleus and a large macronucleus. The first one is responsible for the sexual functions which generate genetic variation and the second provides trophic functions and asexual reproduction

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Diatoms (Arnaud and Hugo)

Diatoms are a major group of algae, and are among the most common types of phytoplankton. Diatoms are unicellular, although they can form colonies in the shape of filaments or ribbons.Only male gametes of centric diatoms are capable of movement.Diatom communities are a popular tool for monitoring environmental conditions, past and present, and are commonly used in studies of water quality.

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Turbidity results (Sophie, Rose, Célia)

Variation of the turbidity in both artificial puddles

We did a graph on the turbidity of the  water which is contained inside and the other  was exposed to the sun light. We can know, with it, that the turbidity increases each day. In the graph, you can see the number “465”, it represents the water which become brown. The number “565” says that the light doesn’t shine the water when the turbidity increases. So to conclude, more turbidity increases, more water turns brown and dark.